Rat Leptin ELISA

RayBio® Rat Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • Q9WVB7
Gene ID:
  • 113917
Gene Symbols:
  • LEP503
Protein Name & Synonyms:
Lens epithelial cell protein LEP503
Target Species:
  • Rat

Assay Format

Detection Range:
30 pg/ml - 8000 pg/ml
Recommended Dilution (serum & plasma):
3 fold
30 pg/ml

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The Leptin ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, L-Selectin, Lix, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha.
Number of Targets Detected:
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Slobadoa N., Feve B., Thornton S., et al. Fatty Acids Impair Endothelium-Dependent Vasorelaxation: A Link Between Obesity and Arterial Stiffness in Very Old Zucker Rats.
    Species: Rat
    Sample type: Plasma
  2. Banerjee, A., Das, D., Paul, R. et al. Mechanistic study of attenuation of monosodium glutamate mixed high lipid diet induced systemic damage in rats by Coccinia grandis. Sci Rep 10, 15443 (2020). https://doi.org/10.1038/s41598-020-72076-6
    Species: Rat
    Sample type: Serum
  3. Govic A., Penman J., Tammer A., Paolini A. Paternal calorie restriction prior to conception alters anxiety-like behavior of the adult rat progeny. Psychoneuroendocrinology. 2016 Feb;64:1-11. doi: 10.1016/j.psyneuen.2015.10.020.
    Species: Rat
    Sample type: Serum (Rat progeny growth studies.)
  4. Thakare M., Surana S. ?-Asarone modulate adipokines and attenuates high fat diet-induced metabolic abnormalities in Wistar rats. Pharmacological Research Volume 103, January 2016, Pages 227-235
    Species: Rat
    Sample type: Serum (High fat induced diet wistar rats)
  5. Ashwini S., Bobby Z., Joseph M., et al. Insulin plant (Costus pictus) extract improves insulin sensitivity and ameliorates atherogenic dyslipidaemia in fructose induced insulin resistant rats: Molecular mechanism. Journal of Functional Foods. Volume 17, August 2015, Pages 749-760
    Species: Rat
    Sample type: Plasma (Fructose induced insulin resistan Rat model)
  6. Mishra, A. K., & Ghosh, A. R. (2020). Probiotic Enterococcus faecalis AG5 mitigated high fat diet induced obesity and produced propionic acid stimulated apoptosis in 3T3-L1 pre-adipocyte. Life Sciences, 118292. doi:10.1016/j.lfs.2020.118292 
    Species: Rat
    Sample type: Serum
  7. Abdel-Reheim E., et al. Fatty-Sucrosed Diet/Minimal Dose of Streptozotocin Treated Rat: A Novel Model of Gestational Diabetes Mellitus, Metabolic and Inflammatory Insight. J Diabetes Metab 2014, 5:9http://dx.doi.org/10.4172/2155-6156.1000430
    Species: Rat
    Sample type: Serum
  8. de Castro G., Seminice R., Simoes-Ambrosio L., et al. Dietary Docosahexaenoic Acid and Eicosapentaenoic Acid Influence Liver Triacylglycerol and Insulin Resistance in Rats Fed a High-Fructose Diet. Mar. Drugs 2015, 13(4), 1864-1881; doi:10.3390/md13041864
    Species: Rat
    Sample type: Serum (High Fructose Diet rats and insulin resistance with treatments)
  9. Zhang Z., Wang Y., Long Y., Wang L., Li Y., Gao F., Tian H. Alteration of sweet taste in high-fat diet induced obese rats after 4 weeks treatment with exenatide. Peptides 47 (2013) 115-123
    Species: Rat
    Sample type: Serum
  10. Saad M., Kamel M., Hanafi M. Modulation of Adipocytokines Production and Serum NEFA Level by Metformin, Glimepiride, and Sitagliptin in HFD/STZ Diabetic Rats. Biochem Res Int. 2015; 2015: 138134. doi: 10.1155/2015/138134
    Species: Rat
    Sample type: Serum (Diabetic rates treated with glimepiride, sitagliptin and HFD/STZ)

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