RayBio® Rat MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.


Antigen Information

Accession Number:
  • P14844
Gene ID:
  • 24770
Gene Symbols:
  • CCL2
  • JE
  • MCP1
  • SCYA2
Protein Name & Synonyms:
C-C motif chemokine 2 (Immediate-early serum-responsive protein JE) (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Small-inducible cytokine A2)
Target Species:
  • Rat

Assay Format

Detection Range:
15 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
500 - 10,000 fold
15 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Number of Targets Detected:
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Smits A., et al. Shear flow affects selective monocyte recruitment into MCP-1-loaded scaffolds. J. Cell. Mol. Med, Early Online view, August 2014. doi: 10.1111/jcmm.12330
    Species: Rat
    Sample type: Conditioned Media
  2. Yang D., Sun YY., Bhaumik SK., et al. Blocking lymphocyte trafficking with FTY720 prevents inflammation-sensitized hypoxic-ischemic brain injury in newborns. J Neurosci. 2014 Dec 3;34(49):16467-81. doi: 10.1523/JNEUROSCI.2582-14.2014
    Species: Rat
    Sample type: Tissue Lysate
  3. Ergenoglu AM, Yeniel AÖ, Erbas O, et al. Regression of Endometrial Implants by Resveratrol in an Experimentally Induced Endometriosis Model in Rats. Reprod Sci. 2013; Published online before print March 27, 2013, doi:10.1177/1933719113483014
    Species: Rat
    Sample type: Serum
  4. Savary-Auzeloux, Isabelle, Hugues Magne, and Carole Migné. "A Dietary Supplementation with Leucine and Antioxidants Is Capable to Accelerate Muscle Mass Recovery after Immobilization in Adult Rats." PLoS One. NCBI, 29 Nov. 2013. Web.
    Species: Rat
    Sample type: Plasma
  5. Yang, Dianer, et al. "Plasminogen activator inhibitor-1 mitigates brain injury in a rat model of infection-sensitized neonatal hypoxia?ischemia."?Cerebral Cortex?(2012): bhs115.
    Species: Rat
    Sample type: Tissue Lysate
  6. Kelley, Rusty, et al. "A population of selected renal cells augments renal function and extends survival in the ZSF1 model of progressive diabetic nephropathy."?Cell transplantation?22.6 (2013): 1023-1039.
    Species: Rat
    Sample type: Urine
  7. Salman, Zenat K., et al. "The combined effect of metformin and L-cysteine on inflammation, oxidative stress and insulin resistance in streptozotocin-induced type 2 diabetes in rats."?European journal of pharmacology?714.1-3 (2013): 448-455.
    Species: Rat
    Sample type: Serum
  8. Schaal SM, Garg MS, Ghosh M, Lovera L, Lopez M, et al. (2012) The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as aNeuroprotectant following Spinal Cord Injury. PLoS ONE 7(9): e43634. doi:10.1371/journal.pone.0043634
    Species: Rat
    Sample type: Tissue Lysate
  9. Yamaleyeva LM, Guimaraes-Souza NK, Krane LS, et al. Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. Stem Cells Translational Medicine. 2012;1(5):373-383. doi:10.5966/sctm.2011-0048
    Species: Rat
    Sample type: Urine
  10. Fu S., Stanek A., Mueller C., et al. Acute pancreatitis in aging animals: loss of pancreatitis-associated protein protection? World J Gastroenterol. 2012 Jul 14;18(26):3379-88. doi: 10.3748/wjg.v18.i26.3379.
    Species: Rat
    Sample type: Tissue Lysate
The kit is wonderful, I used Kidney homogenates.

University of Houston
Excellent results with rat tissue homogenates and serum; easy to use; reproducible

SUNY Downstate Medical Center
Worked well for both plasma and tissue homogenate.

University of Houston
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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