Rat MCP-1 ELISA

RayBio® Rat MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELR-MCP1-1
+

Antigen Information

Accession Number:
  • P14844
Gene ID:
  • 24770
Gene Symbols:
  • CCL2
  • JE
  • MCP1
  • SCYA2
Protein Name & Synonyms:
C-C motif chemokine 2 (Immediate-early serum-responsive protein JE) (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Small-inducible cytokine A2)
Target Species:
  • Rat

Assay Format

Detection Range:
15 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
500 - 10,000 fold
Sensitivity:
15 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wirrig C, Hunter I, Mathieson FA, Nixon GF. Sphingosylphosphorylcholine is a proinflammatory mediator in cerebral arteries. Journal of Cerebral Blood Flow & Metabolism. 2011;31(1):212-221. doi:10.1038/jcbfm.2010.79.
    Species: Rat
    Sample type: Conditioned Media
  2. Chen, Yen-Cheng, et al. "Dipyridamole inhibits lipopolysaccharide-induced cyclooxygenase-2 and monocyte chemoattractant protein-1 via heme oxygenase-1-mediated reactive oxygen species reduction in rat mesangial cells." European journal of pharmacology 650.1 (2011): 445-450.
    Species: Rat
    Sample type: Conditioned Media
  3. Reynolds, Paul R., Karisa M. Wasley, and Camille H. Allison. "Diesel Particulate Matter Induces Receptor for Advanced Glycation End-Products (RAGE) Expression in Pulmonary Epithelial Cells, and RAGE Signaling Influences NF-[kappa] B-Mediated Inflammation." Environmental health perspectives 119.3 (2011): 332.
    Species: Rat
    Sample type: Conditioned Media
  4. Magne H, Savary-Auzeloux I, Vazeille E, et al. Lack of muscle recovery after immobilization in old rats does not result from a defect in normalization of the ubiquitin-proteasome and the caspase-dependent apoptotic pathways. The Journal of Physiology. 2011;589(Pt 3):511-524. doi:10.1113/jphysiol.2010.201707.
    Species: Rat
    Sample type: Tissue lysate
  5. Saleh, M. A., et al. "Endothelin receptor A-specific stimulation of glomerular inflammation and injury in a streptozotocin-induced rat model of diabetes." Diabetologia 54.4 (2011): 979-988.
    Species: Rat
    Sample type: Tissue lysate
  6. Sugimoto, Mikio, et al. "Effect of a phytotherapeutic agent, Eviprostat®, on prostatic and urinary cytokines/chemokines in a rat model of nonbacterial prostatitis." The Prostate 71.4 (2011): 438-444.
    Species: Rat
    Sample type: Urine
  7. Bitar, Milad S., and Fahd Al-Mulla. "A defect in Nrf2 signaling constitutes a mechanism for cellular stress hypersensitivity in a genetic rat model of type 2 diabetes." American Journal of Physiology-Endocrinology and Metabolism 301.6 (2011): E1119-E1129.
    Species: Rat
    Sample type: Conditioned Media
  8. Omi H., et al. Low-intensity pulsed ultrasound stimulation enhances TIMP-1 in nucleus pulposus cells and MCP-1 in macrophages in the rat. J Orthop Res. 2008 Jun;26(6):865-71. doi: 10.1002/jor.20545.
    Species: Rat
    Sample type:
Excellent results with rat tissue homogenates and serum; easy to use; reproducible

SUNY Downstate Medical Center
Worked well for both plasma and tissue homogenate.

University of Houston
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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