Rat MCP-1 ELISA

RayBio® Rat MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$723.60  
ELR-MCP1-CL-2
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Antigen Information

Accession Number:
  • P14844
Gene ID:
  • 24770
Gene Symbols:
  • CCL2
  • JE
  • MCP1
  • SCYA2
Protein Name & Synonyms:
C-C motif chemokine 2 (Immediate-early serum-responsive protein JE) (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Small-inducible cytokine A2)
Target Species:
  • Rat

Assay Format

Detection Range:
15 pg/ml - 18000 pg/ml
Recommended Dilution (serum & plasma):
500 - 10,000 fold
Sensitivity:
15 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Rat
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Yang, Dianer, et al. "Plasminogen activator inhibitor-1 mitigates brain injury in a rat model of infection-sensitized neonatal hypoxia–ischemia." Cerebral Cortex (2012): bhs115.
    Species: Rat
    Sample type: Tissue Lysate (brain lysates)
  2. Reynolds, Paul R., Karisa M. Wasley, and Camille H. Allison. "Diesel Particulate Matter Induces Receptor for Advanced Glycation End-Products (RAGE) Expression in Pulmonary Epithelial Cells, and RAGE Signaling Influences NF-[kappa] B-Mediated Inflammation." Environmental health perspectives 119.3 (2011): 332.
    Species: Rat
    Sample type: Conditioned Media
  3. Saleh, M. A., et al. "Endothelin receptor A-specific stimulation of glomerular inflammation and injury in a streptozotocin-induced rat model of diabetes." Diabetologia 54.4 (2011): 979-988.
    Species: Rat
    Sample type: Serum
  4. Saleh, M. A., et al. "Endothelin receptor A-specific stimulation of glomerular inflammation and injury in a streptozotocin-induced rat model of diabetes." Diabetologia 54.4 (2011): 979-988.
    Species: Rat
    Sample type: Tissue lysate (kidney homogenate)
  5. Yang D., Sun YY., Bhaumik SK., et al. Blocking lymphocyte trafficking with FTY720 prevents inflammation-sensitized hypoxic-ischemic brain injury in newborns. J Neurosci. 2014 Dec 3;34(49):16467-81. doi: 10.1523/JNEUROSCI.2582-14.2014
    Species: Rat
    Sample type: Tissue Lysate (Rat Brain Tissue)
  6. Yamaleyeva LM, Guimaraes-Souza NK, Krane LS, et al. Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. Stem Cells Translational Medicine. 2012;1(5):373-383. doi:10.5966/sctm.2011-0048
    Species: Rat
    Sample type: Tissue Lysate (renal cortex and medulla of ischemia-reperfusion-gentamicin rats.)
  7. Yamaleyeva LM, Guimaraes-Souza NK, Krane LS, et al. Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. Stem Cells Translational Medicine. 2012;1(5):373-383. doi:10.5966/sctm.2011-0048
    Species: Rat
    Sample type: Urine (ischemia-reperfusion-gentamicin rats.)
  8. Yamaleyeva LM, Guimaraes-Souza NK, Krane LS, et al. Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury. Stem Cells Translational Medicine. 2012;1(5):373-383. doi:10.5966/sctm.2011-0048
    Species: Rat
    Sample type: Urine (renal cortex and medulla of ischemia-reperfusion-gentamicin rats.)
  9. Wirrig C, Hunter I, Mathieson FA, Nixon GF. Sphingosylphosphorylcholine is a proinflammatory mediator in cerebral arteries. Journal of Cerebral Blood Flow & Metabolism. 2011;31(1):212-221. doi:10.1038/jcbfm.2010.79.
    Species: Rat
    Sample type: Conditioned Media (A7r5 cells )
  10. Ikutomi M., Sahara M., Nakajima T., et al. Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation. J Mol Cell Cardiol. 2015 Sep;86:121-35. doi: 10.1016/j.yjmcc.2015.07.019.
    Species: Rat
    Sample type: Conditioned Media (Endothelial progenitor cells, Rat endothelial aoritc cells, AoECS, and BM-derived mononuclear cells)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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