RayBio® BrdU Cell Proliferation Kit

137-24008-25
+

Product Specifications

Size:
25 tests or 100 tests

Description

The RayBiotech Cell Proliferation Analyses analyze cellular DNA synthesis and cell cycling in both cell culture and in vivo studies. Cell proliferation is an important physiological and pathological process during homeostasis and disease development (e.g. cancer). Using BrdU as a nucleotide analog to monitor DNA synthesis and cell cycling, provides a precise tool to monitor cell function at single cell level by flow cytometry.

Kit Components

  • BrdU, lyophilized (working stock is 10mg/mL in PBS)
  • Fixation/Permeabilization Buffer
  • Cell Permeabilization Buffer, 10x (dilute with ddH2O to 1x)
  • Nuclear Permeabilization Buffer
  • DNase I, lyophilized (working stock is 500 µg/mL in PBS)
  • RayBright® R647, anti-BrdU antibody, 5µL/test
  • 7-AAD, 50 µg/mL

Product Details

Conjugate/Tag/Label
RayBright® Live R647
Excitation Laser
Red Laser, 633 nm
Maximum Emission Wavelength
664 nm, equivalent to Cy5™ and Alexa Fluor™ 647 (Cy5™ and Alexa Fluor™ are the trademark of Thermo Fisher Scientific and GE Health Care)

Storage/Stability

BrdU and Dnase I (after reconstitution) should be frozen as aliquots at -80°C, avoid frequent freeze/thaw. Thawed BrdU solutions are stable for up to 4 weeks at 4°C. other components can be stored at 4°C, protected from light for up to 3 months

Product Analysis

One million Jurkat cells were labeled with 10 µM BrdU and stained with RayBright® R647 anti-BrdU and 7-AAD (see manual for details).

One million Jurkat cells were labeled with 10 µM BrdU and stained with RayBright® R647 anti-BrdU and 7-AAD (see manual for details).

Note

Note: RayBright® is a trademark of RayBiotech, Inc.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.