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Peptide Array-Based Epitope Mapping Service
Introduction
Epitope mapping is a method that is often employed to identify the antigen binding sites of an antibody. However, epitope mapping can also be used in other protein interactions with high affinity, including those with proteins, chemicals, and DNA. RayBiotech’s peptide array-based epitope mapping service uses high-quality peptides that are chemically synthesized, thereby negating many of the steps and time required with the traditional approach that involves recombinant cloning, expression, and purification of different regions of the target gene.
RayBiotech’s comprehensive epitope mapping service includes peptide design and synthesis, array printing, sample testing, and report generation. Just send us your samples, and we’ll send you the report!
Applications
- Identify high antigenicity epitopes for antibody production & vaccine development
- Locate (auto)antigen epitopes of (auto)antibodies
- Define the best antibody clone against the protein-of-interest
- Perform epitope mutant analysis
- Map B cell epitopes
Service Features
- Small serum sample volume required (2 µl)
- High density (simultaneously analyze hundreds of overlapping peptides)
- High sensitivity
- Large dynamic range
- Suitable for high-throughput assays
- High efficiency and accuracy
- Affordable, quick and simple to use
Pre-Designed Epitope Mapping Arrays
In addition to custom epitope mapping arrays, RayBiotech offers pre-designed peptide panels for popular drug targets. For more information, please contact our technical support team at [email protected].
Cytotoxic T-Lymphocyte Associated Protein 4 (CTLA4)
Lymphocyte Activating 3 (LAG3)
Programmed Cell Death 1 (PD-1)
Programmed Death Ligand 1 (PD-L1)
Insulin
Interleukin 2 (IL-2)
Tumor necrosis factor alpha (TNF-alpha)
How It Works
RayBiotech’s peptide-based epitope mapping arrays are prepared by first printing overlapping, chemically-synthesized peptides (> 95% purity) spanning the length of the protein-of-interest onto a glass slide surface in an arrayed format, with each peptide spotted in triplicate. Multiple positive and negative controls are also printed onto the array to monitor each incubation step. Purified antibodies or bodily fluids containing antibodies are then diluted and incubated on the peptide arrays, during which the antibodies specifically bind to the peptides displaying their sequence-of-interest. After unbound antibodies are washed off the array, a biotin-conjugated secondary antibody (e.g., anti-human IgG, mouse IgG) and a fluorescence dye-conjugated streptavidin molecule are added to the array to enable the detection of bound antibodies bound. Fluorescent signal is then captured using a laser scanner. Since the peptide sequence of each spot is known, the antibody’s potential epitope(s) can be ascertained.
Array Format
- Number of replicates per peptide: Triplicate
- Array substrate: Glass slide
- Number of subarrays per slide: 4
- Printed controls: Biotin-BSA, human IgG, mouse IgG, full-length target protein (unless otherwise specified)
Assay Format
- Compatible Sample Types: Antibodies (serum, plasma, ascites, recombinant antibodies, purified antibodies), chemicals, aptamer, or any other binders.
- Antibody Type: Human IgG and mouse IgG. Other isotypes (IgA, IgM, IgE, etc.) derived from most animals, upon request.
- Method of Detection: Fluorescence captured by laser scanner
- Data: Semi-Quantitative
Representative Data
Results are presented as a fluorescent scan of the array (Figure 1) and in a table format (Table 1).
RayBiotech would like to thank Dr. Clemens Duerrschmid (Baylor College of Medicine, Houston, TX) for allowing us to present this data.
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RayBiotech Life, Inc.
3607 Parkway Lane Suite 200
Peachtree Corners, GA 30092
Tel: 770-729-2992, 1-888-494-8555 (toll-free)
Fax: 770-206-2393
Email: [email protected]
