Peptide Array-Based Epitope Mapping Service

Applications

• Identify high antigenicity epitopes for antibody production & vaccine development
• Locate (auto)antigen epitopes of (auto)antibodies
• Define the best antibody clone against the protein-of-interest
• Perform epitope mutant analysis
• Map B cell epitopes

Service Features

• Small serum sample volume required (2 µl)
• High density (simultaneously analyze hundreds of overlapping peptides)
• High sensitivity
• Large dynamic range
• Suitable for high-throughput assays
• High efficiency and accuracy
• Affordable, quick and simple to use

Available Pre-made Epitope Mapping Arrays

In addition to custom epitope mapping arrays, RayBiotech offers pre-made arrays for popular targets. For more information, please contact our technical support team at [email protected].

How It Works

RayBiotech’s peptide-based epitope mapping arrays are prepared by first printing overlapping, chemically-synthesized peptides (> 95% purity) spanning the length of the protein-of-interest onto a glass slide surface in an arrayed format, with each peptide spotted in triplicate. Multiple positive and negative controls are also printed onto the array to monitor each incubation step. Purified antibodies or bodily fluids containing antibodies are then diluted and incubated on the peptide arrays, during which the antibodies specifically bind to the peptides displaying their sequence-of-interest. After unbound antibodies are washed off the array, a biotin-conjugated secondary antibody (e.g., anti-human IgG, mouse IgG) and a fluorescence dye-conjugated streptavidin molecule are added to the array to enable the detection of bound antibodies bound. Fluorescent signal is then captured using a laser scanner. Since the peptide sequence of each spot is known, the antibody’s potential epitope(s) can be ascertained.

Array Format

• Number of replicates per peptide: Triplicate
• Array substrate: Glass slide
• Number of subarrays per slide: 4
• Printed controls: Biotin-BSA, human IgG, mouse IgG, full-length target protein (unless otherwise specified)

Assay Format

• Compatible Sample Types: Antibodies (serum, plasma, ascites, recombinant antibodies, purified antibodies), chemicals, aptamer, or any other binders.
• Antibody Type: Human IgG and mouse IgG. Other isotypes (IgA, IgM, IgE, etc.) derived from most animals, upon request.
• Method of Detection: Fluorescence captured by laser scanner
• Data: Semi-Quantitative

Representative Data

Results are presented as a fluorescent scan of the array (Figure 1) and in a table format (Table 1).

RayBiotech would like to thank Dr. Clemens Duerrschmid (Baylor College of Medicine, Houston, TX) for allowing us to present this data.