RayBright® Violet 450 Anti-Mouse CD3

Clone: 145-2C11

135-08011-025
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Antigen Information

Gene Symbols:
  • CD3
Protein Name & Synonyms:
CD3
Target Species:
  • Mouse

Antibody Specifications

Applications:
Flow Cytometry
Clone:
145-2C11
Concentration:
0.5 mg/ml
Conjugation/Tag:
RayBright® Violet 450
Host Species:
Hamster
Preparation:
The antibody was purified by affinity chromatography, and conjugated with a high quality fluorochrome, RayBright® Violet 450
Species Reactivity:
Mouse
Isotype:
Hamster IgG1, k

Typical Data

Description

CD3ε, a subunit of CD3, is a 20 kDa transmembrane protein primarily expressed on pre-mature thymocytes, peripheral mature T cells, and NK-T cells. The CD3 molecules consist of CD3ε, δ,γ, and ζ chains and form a TCR complex by associating with TCR α/β orγ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding with the peptide/MHC antigen complex. The TCR on the T cell surface is non-covalently associated with CD3 subunits: CD3εγ and CD3εδ heterodimers and the CD3ζζ homodimer, which collectively form the TCR-CD3 complex. Antigen presentation of peptide-loaded MHC molecules by APCs, along with other co-stimulation signals, leads to activation of T cells. This antibody can recognize mouse CD3ε. It can also activate T cells in vitro together with anti-mouse CD28.

Product Details

Excitation Laser
Violet Laser, 407 nm
Maximum Emission Wavelength
454 nm, replace Pacific Blue™
Buffer
PBS, PH7.2~7.4, 0.2% BSA, 0.1% NaN3
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per 106 cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage/Stability
Stored at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.