RayPlex Human Inflammation Array 1

RayPlex Human Inflammation Array 1 Kit. Detects 13 Human Inflammatory Factors. Suitable for serum, conditioned media, cell lysate, tissue lysate, and other liquid sample types.

$942.50  
FAH-INF-1-50
+

Antigen Information

Gene Symbols:
CCL2 | CSF3 | IFNG | IL10 | IL12A | IL13 | IL17A | IL1B | IL2 | IL23A   View more

Assay Format

Number of Targets Detected:
13
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Flow Cytometry
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Fluorescent Microbeads

Product Specifications

Size:
50, 100, 200, or 500 tests (Each sample replicate is considered a test. 16 tests will be used to generate the standard curve.)

Product Features

  • Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
  • 4 hour processing time
  • 5-10x more cost-effective than ELISA
  • Compatible with most flow cytometers
  • No specialized or dedicated instrument(s) needed
  • High specificity and system reproducibility
  • Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
GCSF
IFN-gamma
IL-10
IL-12 p70
IL-13
IL-17A
IL-1 beta
(IL-1 F2)
IL-2
IL-23 p19
IL-4
IL-6
MCP-1
(CCL2)
TNF alpha
   

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • RayPlex Multiplex Bead Cocktail
  • 1X Assay Diluent
  • 20X Wash Buffer
  • Lyophilized Protein Standard Mix
  • 10X Biotinylated Detection Antibody Cocktail
  • 100X Streptavidin-PE
  • Rainbow Particles
  • Extra RayPlex Multiplex Bead Cocktail
  • V-bottom 96-well Microplate
  • Filter 96-well Microplate
  • RayBio® Serum Diluent
Other Materials Required
  • Orbital 96-well plate shaker (with ability to reach 1000 rpm)
  • Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
  • Aluminum foil or 96-well Plate Aluminum Sealers
  • Distilled water
  • 1.5 ml polypropylene microcentrifuge tubes or similar
  • Microcentrifuge
  • If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
  • Optional: 96-well round bottom plate (to prepare samples)
  • Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex bead arrays. Get a quote!

Protocol Outline
  1. Prepare Reagents, Standards, and Samples
  2. Add RayPlex Multiplex Bead Cocktail to each well
  3. Incubate with Samples and Standards
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluorophore
  6. Analyze with a compatible flow cytometer
  7. Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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