RayPlex™ Human Inflammation Array 1

RayPlex™ Human Inflammation Array 1 Kit. Detects 13 Human Inflammatory Factors. Suitable for serum, conditioned media, cell lysate, tissue lysate, and other liquid sample types.

$1,450.00  
FAH-INF-1-96
+

Assay Format

Number of Targets Detected:
13
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Flow Cytometry
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Fluorescent Microbeads

Product Specifications

Size:
48, 96, 192, or 480 tests (Each sample replicate is considered a test. 16 tests will be used to generate the standard curve.)

Protein Information

Gene Symbols:
CCL2 | CSF3 | IFNG | IL10 | IL12A | IL13 | IL17A | IL1B | IL2 | IL23A   View more

Product Features

  • Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
  • 4 hour processing time
  • 5-10x more cost-effective than ELISA
  • Compatible with most flow cytometers
  • No specialized or dedicated instrument(s) needed
  • High specificity and system reproducibility
  • Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
GCSF
IFN-gamma
IL-10
IL-12 p70
IL-13
IL-17A
IL-1 beta
(IL-1 F2)
IL-2
IL-23 p19
IL-4
IL-6
MCP-1
(CCL2)
TNF alpha
  

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • RayPlex™ Multiplex Bead Cocktail
  • 1X Assay Diluent
  • 20X Wash Buffer
  • Lyophilized Protein Standard Mix
  • 10X Biotinylated Detection Antibody Cocktail
  • 10X Streptavidin-PE
  • Flow Cytometer Setup Bead Cocktail
  • V-bottom 96-well Microplate
  • Filter 96-well Microplate
  • RayBio® Serum Diluent
Other Materials Required
  • Orbital 96-well plate shaker (with ability to reach 1000 rpm)
  • Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
  • Aluminum foil or 96-well Plate Aluminum Sealers
  • Distilled water
  • 1.5 ml polypropylene microcentrifuge tubes or similar
  • Microcentrifuge
  • If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
  • Optional: 96-well round bottom plate (to prepare samples)
  • Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline
  1. Prepare Reagents, Standards, and Samples
  2. Add RayPlex™ Multiplex Bead Cocktail to each well
  3. Incubate with Samples and Standards
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluorophore
  6. Analyze with a compatible flow cytometer
  7. Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.