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| CODE | RayPlex Size | Price | ||
|---|---|---|---|---|
|
FAH-INF-1-48
|
$943.00 | ||
|
FAH-INF-1-96
|
$1,450.00 | ||
|
|
FAH-INF-1-192
|
$2,683.00 | ||
|
|
FAH-INF-1-480
|
$6,163.00 |
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Assay Format
Number of Targets Detected:
13
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Flow Cytometry
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
Fluorescent Microbeads
Product Specifications
Size:
48, 96, 192, or 480 tests (Each sample replicate is considered a test. 16 tests will be used to generate the standard curve.)
Protein Information
Gene Symbols:
Product Features
- Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
- 4 hour processing time
- 5-10x more cost-effective than ELISA
- Compatible with most flow cytometers
- No specialized or dedicated instrument(s) needed
- High specificity and system reproducibility
- Well-suited for high throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GCSF
| IFN-gamma
| IL-10
| IL-12 p70
| IL-13
|
IL-17A
| IL-1 beta (IL-1 F2)
| IL-2
| IL-23 p19
| IL-4
|
IL-6
| MCP-1 (CCL2)
| TNF alpha
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- RayPlex Multiplex Bead Cocktail
- 1X Assay Diluent
- 20X Wash Buffer
- Lyophilized Protein Standard Mix
- 10X Biotinylated Detection Antibody Cocktail
- 10X Streptavidin-PE
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Filter 96-well Microplate
- RayBio® Serum Diluent
Other Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- 1.5 ml polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex bead arrays. Get a quote!
Protocol Outline
- Prepare Reagents, Standards, and Samples
- Add RayPlex Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
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