Recombinant Actinidia Deliciosa (Kiwi) Act d 5

Recombinant Actinidia deliciosa (kiwi) Act d 5 derived from E.coli

$90.92
230-00058-10
+

Source

Gene Symbols:
  • Act d 5
Species:
Actinidia deliciosa (Kiwi)
Accession Number:
  • P84527
Expressed Region:
Ile1-Ala189
Protein Name & Synonyms:
Act d 5, Kiwellin.

Preparation

Expression System:
E.Coli
Conjugation/Tag:
N-terminal histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
>95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
21 kDa (the actual purified protein band is ~14 kDa, smaller than expected for some unknown reasons. DNA construct sequencing is correct.)

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.45 ?m filtered solution in 1x PBS (pH 7.4).
Concentration:
Determined by Pierce BCA protein assay kit
SDS-PAGE Image

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.