Recombinant Anti-C. botulinum Toxin B with Anti-Myc Tag

Recombinant Anti-C. botulinum Toxin B Antibody

$51.51  
RB-05-0002-20
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Antibody Specifications

Applications:
ELISA (recommended work dilution= 1: 1,000-2,000), Western Blotting (recommended work dilution= 1~5 µg/mL), Dot Blotting(recommended work dilution= 1~5 µg/mL). Anti-Myc Tag Antibody Applications: Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)
Concentration:
2.7 mg/ml
Preparation:
Antigen: Botulinum Neurotoxin Type B, ToxoidGeneration: Human recombinant antibody to Toxin B was expressed in E. coli and purified using NiNTA affinity chromatography. The antibody is tagged with a myc-tag (EQKLI SEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.Purification: Metal chelate affinity chromatography
Species Reactivity:
RB-05-0002 specifically detects Toxin B as determined from testing bacterial samples in Dot Blot and ELISA assays.

Description

Clostridium botulinum is a Gram-positive, rod shaped bacterium that produces the neurotoxin botulin, which causes the flaccid muscular paralysis seen in botulism, and is also the main paralytic agent in botox. It is an anaerobic spore-former, which produces oval, subterminal endospores and is commonly found in soil. It is an obligate anaerobe, meaning that oxygen is poisonous to the cells. Since 1953 all species producing the botulinum neurotoxins (types A-H) have been identified from C. botulinum. The neurotoxins produced by the eight types of Clostridium botulinum act primarily at the neuromuscular junction and cause the syndrome botulism, which is frequently a fatal condition in humans and animals.

Specificity

RB-05-0002 specifically detects Toxin B as determined from testing bacterial samples in Dot Blot and ELISA assays.
Anti-Myc Tag Antibody Applications
Anti-Myc Tag Antibody Applications
Immunoassay (ELISA, Western blotting)(recommended work dilution= 1:2,000-1:5,000)

Storage

Stable for up to one year at -20 °C to -70 °C from date of receipt. After thawing, this product can be stored at 2 - 8 °C for at least one month without detectable loss of activity. Please avoid freeze-thaw cycles.

References

  • C. L. Hatheway, L. M. McCroskey. 1987. Examination of faeces for diagnosis of infant botulism in 336 patients. J. Clin. Microbiol. 25: 2334–2338.
  • Hauschild, A.H.W. 1989. Clostridium botulinum. In M. P. Doyle (ed.), Food-borne Bacterial Pathogens. Marcel Dekker, New York. Pp. 111–189
  • B Syuto and S Kubo. 1977. Isolation and molecular size of Clostridium botulinum type C toxin. Appl Environ Microbiol. 33(2): 400–405.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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