Tris with NP40, Sodium Deoxycholate and PMSF.
Concentration (lot specific):
Total Protein: 24.14 mg/mL (Dye Binding Assay)
Purity determined by:
Chronically infected cells are harvested by centrifugation and disrupted by sonication and cell debris is removed by centrifugation.
This product should be sonicated immediately prior to use to ensure that the preparation is uniform. This preparation may be used in a variety of immunoassay formats including microwell ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Detergent and sonication.
Upon receipt, store at ?-70°C. Avoid multiple freeze/thaw cycles.