Purification is conducted from broken yeast cells by the following scheme: clarification, microfiltration, ultrafiltration, adsorption chromatography, ion-exchange chromatography, ultracentrifugation and sterile filtration. Product is sterile filtered.
0.05 M Phosphate, 0.2 M Sodium Chloride, pH 7.2.
1.97 mg/mL (Lowry)
EIA Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.