Recombinant Human ALK tyrosine kinase receptor

Recombinant Human Anaplastic Lymphoma Kinase (ALK / p80)
CODE:  230-00014

Source

Gene Symbols:
  • ALK
Species:
Human
Accession Number:
  • NP_004295
Expressed Region:
Hybrid polypeptide (Phe419-Val526, Pro650-Thr680 and Glu1605-Pro1620)
Protein Name & Synonyms:
ALK, anaplastic lymphoma receptor tyrosine kinase, anaplastic lymphoma kinase, CD246, ALK tyrosine kinase receptor.

Preparation

Expression System:
E.Coli
Conjugation/Tag:
N-terminal 6x histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
20

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered PBS solution with the protein concentration of 0.5 mg/mL.
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.
Endotoxin Level:
Not determined
Preservative:
None

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.