Lyophilized from a 0.2 um filtered PBS solution with the protein concentration of 0.5 mg/mL.
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.
Endotoxin Level:
Not determined
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Preservative:
None
Recommended Applications:
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array
Source
Gene Symbols:
ALK
Species:
Human
Accession Number:
NP_004295
Expressed Region:
Hybrid polypeptide (Phe419-Val526, Pro650-Thr680 and Glu1605-Pro1620)
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.