3607 Parkway Lane Suite 200
Peachtree Corners, GA 30092
Email: [email protected]
Recombinant Human Angiotensin-converting Enzyme 2 (ACE2)
Recombinant Human Angiotensin-converting Enzyme 2 (ACE2) with C-terminal His-tag, derived from the transfected human HEK293 cells.
<div class="text-right"><a href="/files/datasheet/230-30165.pdf" target="_blank" rel="noopener"><img src="/files/images/Buttons/pdf.png" alt="" /> Datasheet</a></div>
100 ug, 500 ug, or 1000 ug
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Lot specific (see the label on the vial), determined by BCA protein assay
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Binding assay, enzyme activity assay, ELISA, glycosylation analysis, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, etc.
Recombinant protein product has a calculated molecular mass of ∼83 kDa. Due to the abundant glycosylation, it migrates as approximately 90 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Stability & Storage
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.