Protein Name & Synonyms:
>98% by SDS PAGE/HPLC analysis
Molecular Weight (kDa):
Purified recombinant protein - lyophilized
Total protein concentration ~30ug/ml after reconstitution
In vitro Assay
Annexin V is a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis.
Detection of PS using Annexin V provides a reliable diagnostic tool for the indication of early programmed cell death. RayBiotech recommends the use of Annexin V assay kits for this purpose.
Annexin V has also been identified as an inhibitor of prothrombin activation in the blood coagulation cascade, and the presence of antibodies to Annexin V are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc).
20mM Phosphate Buffer
0.02% Tween 80
130mM Arginine HCl.
Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for6 months from date of reconstitution.