Recombinant Human BCEI / TFF1

$90.92

Recombinant Breast cancer estrogen-inducible protein (BCEI) / Trefoil Factor 1 / TFF1, expressed in E. coli



230-00253-10
+

Source

Gene Symbols:
  • TFF1
Species:
Human
Accession Number:
  • P04155
Gene ID:
  • 7031
Expressed Region:
Glu25-Phe84
Protein Name & Synonyms:
Breast cancer estrogen-inducible protein /Trefoil factor 1

Preparation

Expression System:
E.Coli
Conjugation/Tag:
N-terminal 6x histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
10

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 µ,m filtered solution in 100 mM NaH2PO4, 10 mM Tris-HCl, 100 mM imidazole, 1% sucrose solution(pH 8.0) with the protein concentration of 781 µ,g/ml
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Preservative:
None

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.