Recombinant Human Bcl2-L-1

Recombinant Human Bcl2-L-1, derived from the transfected human HEK293 cells.

$130.52
230-30068-10
+

Source

Gene Symbols:
  • BCL2L1
Species:
Human
Accession Number:
  • Q07817
Expressed Region:
Ser2-Arg209
Protein Name & Synonyms:
Bcl2-L-1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2 Like 1, Protein Phosphatase 1, Regulatory Subunit 52, BCL2L, BCLX, BCL-XL/S, PPP1R52, Bcl-X

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation/Tag:
N-terminal histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
>95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant Human Bcl2-L-1 has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 µm filtered solution in PBS
Concentration:
Determined by Pierce BCA protein assay kit
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Preservative:
None
SDS-PAGE Image

Stability & Storage

Shipping
Ice packs. Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.