Bcl2-L-1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2 Like 1, Protein Phosphatase 1, Regulatory Subunit 52, BCL2L, BCLX, BCL-XL/S, PPP1R52, Bcl-X
Human Embryonic Kidney 293 Cells
N-terminal histidine tag
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant Human Bcl2-L-1 has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Lyophilized from a 0.2 µm filtered solution in PBS
Determined by Pierce BCA protein assay kit
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Stability & Storage
Ice packs. Including one vial of cell culture supernatant of HEK293 cells transfected with empty expression vector.
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.