Recombinant Human BNP-32
Recombinant human BNP-32, derived from the transfected human HEK293 cells.
10 ug, 50 ug, or 100 ug
Protein Name & Synonyms:
BNP-32, Natriuretic peptides B, Natriuretic Peptide B, Brain Type Natriuretic Peptide, Natriuretic Peptide Precursor B, Gamma-Brain Natriuretic Peptide, Natriuretic Protein, BNP
Human Embryonic Kidney 293 Cells
N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant human BNP-32 has a calculated molecular mass of 30 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 37 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Lyophilized from a 0.2 um filtered solution in PBS
Determined by BCA protein assay kit (Thermo Scientific)
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.