His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Lyophilized from a 0.2 µmicro,m filtered 0.1 M NaHCO3, 0.5 M NaCl, 1% sucrose (pH 8.3) solution with the protein concentration of 0.29 mg/mL
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Stability & Storage
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.