Lyophilized from a 0.2 um filtered solution in PBS
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ELISA, EIA, protein-protein interaction studies, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array
Protein Name & Synonyms:
CDC28 protein kinase regulatory subunit 1B
N-terminal 6x histidine tag
Molecular Weight (kDa):
Stability & Storage
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.