Recombinant Human GPBB / PYGB

Recombinant Human Glycogen Phosphorylase Isoenzyme BB, brain specific (GPBB) / PYGB

$90.92
230-00042-10
+

Source

Gene Symbols:
  • PYGB
Species:
Human
Accession Number:
  • P11216
Gene ID:
  • 5834
Expressed Region:
Val650 - Asp843
Protein Name & Synonyms:
Glycogen phosphorylase, brain form, glycogen phosphorylase B.

Preparation

Expression System:
E.Coli
Conjugation/Tag:
N-terminal 6x histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
22

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered 50 mM Tris-HCl (pH 7.4) solution with the protein concentration of 1.2 mg/mL.
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.
Endotoxin Level:
Not determined
Preservative:
None

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.