Recombinant Human HE4

Recombinant human HE4 protein, derived from transfected HEK293 cells

$130.52
230-30001-10
+

Source

Gene Symbols:
  • WFDC2
Species:
Human
Accession Number:
  • Q14508
Gene ID:
  • 10406
Expressed Region:
Glu31-Phe124
Protein Name & Synonyms:
WAP four-disulfide core domain protein 2, epididymal secretory protein E4, WAP5

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation/Tag:
N-terminal 8x histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
The predicted size of HE4 protein is 12 kDa. The affinity purified recombinant human HE4 showed two bands: the major band is about 20 kDa, and the minor band is about 12 kDa. Both bands were confirmed by Western blotting using anti-HE4 antibody. The 20-kDa band may represent N-glycosylated form (Ronny Drapkin, et al. Cancer Res 2005, 65(6): 2162-9).

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 µmicro,m filtered solution in PBS.
Concentration:
Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Preservative:
None
SDS-PAGE Image

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.

References

  • Ryan M. Williams, Christopher Lee, Thomas V. Galassi, et al. Noninvasive ovarian cancer biomarker detection via an optical nanosensor implant. Science Advances. 18 Apr 2018: Vol. 4, no. 4. DOI: 10.1126/sciadv.aaq1090

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.