Recombinant Human Hepatocyte Growth Factor (HGF), Biotinylated

Biotin-conjugated recombinant Human HGF, derived from the transfected human HEK293 cells

$299.00  
230-30011B-50
+

Product Specifications

Size:
10 ug or 50 ug
Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific):
Determined by BCA protein assay kit (Thermo Scientific)
Purity:
>90%
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Preservative:
None
Recommended Applications:
ELISA, EIA, protein-protein interaction studies, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array
Protein Type:
Recombinant Protein

Source

Gene Symbols:
  • HGF
Species:
Human
Accession Number:
  • P14210
Gene ID:
  • 3082
Expressed Region:
Gln32-Ser728
Protein Name & Synonyms:
Hepatocyte Growth Factor, F-TCF, DFNB39, HGFB, Scatter Factor, Hepatocyte Growth Factor, Fibroblast-Derived Tumor Cytotoxic Factor, hepatopoeitin-A, Lung Fibroblast-Derived Mitogen, HPTA, Hepatopoietin-A, SF Deafness, Autosomal Recessive 39, Hepatopoietin A

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation:
Biotin
Tag:
His
Conjugation/Tag Notes:
N-terminal 8x histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method.
Molecular Weight (kDa):
80
SDS-PAGE Image
SDS-PAGE

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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