Recombinant human IGFBP-2, transfected HEK293 cell lysate

Recombinant human Insulin-like growth factor-binding protein 2 overexpression cell lysate, derived from the transfected HEK293 cells (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (catalog #. 230-10006) transfected with empty expression vector.

$146.01  
230-10278-100
+

Product Specifications

Size:
100 ug
Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Purification:
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Concentration:
Determined by Pierce BCA protein assay kit
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Recommended Applications:
Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein functional assay, protein-protein interaction, post-translational modifications, etc.

Source

Gene Symbols:
  • IGFBP2
Species:
Human
Accession Number:
  • P18065
Expressed Region:
Ala36-Gln325
Protein Name & Synonyms:
IGFBP-2, transfected HEK293 cell lysate, Insulin Like Growth Factor Binding Protein 2, Insulin-Like Growth Factor Binding Protein 2, 36kDa, IGF-Binding Protein 2, IBP2, Insulin-Like Growth Factor Binding Protein 2 (36kD), Insulin-Like Growth Factor Binding Protein 2, Insulin-Like Growth Factor-Binding Protein 2, IGF-BP53, IGFBP-2, IBP-2, BP2.

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation/Tag:
N-terminal histidine tag
Molecular Weight (kDa):
Recombinant protein has a calculated molecular mass of 31. The actual molecular weight may increase differently due to the potential post-modifications (PTMs).

Stability & Storage

Shipping
Ice packs. Including one vial of HEK293 cell lysate transfected with empty expression vector.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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