Recombinant Human Insulin-like growth factor I (IGF-I)

$130.52

Recombinant human IGF-I (Insulin-like growth factor 1, somatomedin C), derived from the transfected human HEK293 cells.



230-30030-10
+

Source

Gene Symbols:
  • IGF1
Species:
Human
Accession Number:
  • P05019
Gene ID:
  • 3479
Expressed Region:
Gly49-Lys195
Protein Name & Synonyms:
Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C, Insulin-Like Growth Factor 1, IBP1, IGFI

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation/Tag:
N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 90%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant mouse IGF-I has a calculated molecular mass of 43 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 43-45 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 µ,m filtered solution in PBS
Concentration:
Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Preservative:
None
SDS-PAGE Image

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.