Recombinant Human Insulin-like growth factor I (IGF-I)
Recombinant human IGF-I (Insulin-like growth factor 1, somatomedin C), derived from the transfected human HEK293 cells.
Protein Name & Synonyms:
Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C, Insulin-Like Growth Factor 1, IBP1, IGFI
Human Embryonic Kidney 293 Cells
N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant mouse IGF-I has a calculated molecular mass of 43 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 43-45 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Lyophilized from a 0.2 um filtered solution in PBS
Determined by BCA protein assay kit (Thermo Scientific)
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.