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Recombinant Human Interleukin-18 (IL-18)

Recombinant human interleukin-18 (IL-18) derived from E.coli

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230-00229-10
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Source

Gene Symbols:
  • IL18
Species:
Human
Accession Number:
  • Q14116
Expressed Region:
Tyr37-Asp193
Protein Name & Synonyms:
Interleukin 18 (interferon-gamma-inducing factor), IL-18.

Preparation

Expression System:
Escherichia coli (E.coli)
Conjugation/Tag:
N-terminal histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
18

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS (pH 7.4)
Concentration:
Determined by Bradford protein assay
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1-1 mg/mL with sterile deionized water.
SDS-PAGE Image
SDS-PAGE

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.