Recombinant Human MIF
Recombinant Human Macrophage Migration Inhibitory Factor / MIF
Supplied as a 0.2 um filtered solution of phosphate-buffered saline (PBS) containing 30% glycerol.
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Lot specific (see the label on the vial)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, etc. Before use, heat the WBC control at 95 °,C for 5 minutes. Spin down the sample and load 5 µ,L per lane onto SDS-PAGE gel.
Protein Name & Synonyms:
MIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, GLIF, glycosylation inhibiting factor, phenylpyruvate tautomerase.
N-terminal 6x histidine tag
Molecular Weight (kDa):
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.