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Recombinant Human NT-proBNP

Recombinant human NT-proBNP, derived from the transfected human HEK293 cells.

$130.52  
230-30019-10
+

Product Specifications

Size:
10 ug, 50 ug, or 100 ug

Source

Gene Symbols:
  • NPPB
Species:
Human
Accession Number:
  • P16860
Gene ID:
  • 4879
Expressed Region:
His27-Arg102
Protein Name & Synonyms:
NT-proBNP, Natriuretic peptides B, Natriuretic Peptide B, Brain Type Natriuretic Peptide, Natriuretic Peptide Precursor B, Gamma-Brain Natriuretic Peptide, Natriuretic Protein, BNP

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation/Tag:
N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 90%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant human NT-proBNP has a calculated molecular mass of 35 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 40-50 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS
Concentration:
Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Preservative:
None
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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