Recombinant Human PCNA
Recombinant Human Proliferating Cell Nuclear Antigen (PCNA)
10 ug, 50 ug, or 100 ug
Protein Name & Synonyms:
Proliferating cell nuclear antigen, PCNA, DNA polymerase delta auxiliary protein, cyclin, proliferating cell nuclear antigen.
N-terminal 6x histidine tag
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Lyophilized from a 0.2 um filtered phosphate-buffered saline (PBS) with protein concentration at 0.8 mg/mL.
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.