Protein Name & Synonyms:
>98% by SDS PAGE and HPLC analysis
Molecular Weight (kDa):
24.3kD (218 amino acid sequence)
Purified recombinant protein - lyophilized
10 mM Acetic Acid
Total protein concentration ~0.1 mg/ml after reconstitution.
ELISA (0.2-0.4ng/well), Functional Assays (0.2ng/ml-10ng/ml), Western Blotting (1.5-3.0ng/lane)
DS-01-0489 is an E. coli-derived recombinant protein corresponding to mature full-length human Platelet-Derived Growth Factor BB homodimer (PDGF-BB), a potent mitogen for cells of mesenchymal origin. PDGF is composed of a dimer of two chains, known as the A chain and B chain. The dimer may present as either AA or BB homodimer, or as an AB heterodimer.
DS-01-0489 has an ED50 of 1.0-3.0 ng/ml as determined by stimulation of the proliferation of Balb/c 3T3 cells.
Prior to reconstitution store at +4°C. Following reconstitution store at -20°C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.