Recombinant Human PDGF BB HOMODIMER

$488.00
DS-01-0489
+

Source

Species:
Human
Protein Name & Synonyms:
PDGF,

Preparation

Purity:
>98% by SDS PAGE and HPLC analysis
Molecular Weight (kDa):
24.3kD (218 amino acid sequence)

Protein Specifications

Format:
Purified recombinant protein - lyophilized
Formulation:
10 mM Acetic Acid
Concentration:
Total protein concentration ~0.1 mg/ml after reconstitution.
Endotoxin Level:
<0.1ng/ug
Preservative:
None present
Recommended Applications:
ELISA (0.2-0.4ng/well), Functional Assays (0.2ng/ml-10ng/ml), Western Blotting (1.5-3.0ng/lane)

Description

DS-01-0489 is an E. coli-derived recombinant protein corresponding to mature full-length human Platelet-Derived Growth Factor BB homodimer (PDGF-BB), a potent mitogen for cells of mesenchymal origin. PDGF is composed of a dimer of two chains, known as the A chain and B chain. The dimer may present as either AA or BB homodimer, or as an AB heterodimer.

DS-01-0489 has an ED50 of 1.0-3.0 ng/ml as determined by stimulation of the proliferation of Balb/c 3T3 cells.

Specifications

Size
10 ug

Stability & Storage

Storage/Stability
Prior to reconstitution store at +4°C. Following reconstitution store at -20°C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.