Recombinant Human Retinol Binding Protein 4 (RBP4)

Recombinant human retinol binding protein 4 (RBP 4), derived from the transfected human HEK293 cells.

$90.92
230-30123-10
+

Source

Gene Symbols:
  • RBP4
Species:
Human
Accession Number:
  • P02753
Expressed Region:
Glu19-Leu201
Protein Name & Synonyms:
Retinol Binding Protein 4, Plasma Retinol-Binding Protein, PRBP, RBP4

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Conjugation/Tag:
C-terminal his-tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
>95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 20 kDa. Due to the potential post-translational modifications, it migrates as approximately 25 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS (pH 7.4)
Concentration:
Determined by BCA protein assay
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
SDS-PAGE Image
SDS-PAGE

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.