Recombinant Human SERPINA1
Recombinant Human alpha-1-Antitrypsin (AAT) / SERPINA1, expressed in E. coli
Protein Name & Synonyms:
Alpha-1-antitrypsin, Serpin A1, AAT, PI1
N-terminal 6x histidine tag
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Lyophilized from a 0.2 um filtered PBS (pH 7.4) containing 1% sucrose solution with the protein concentration of 139 ug/ml
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X❏, Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.