Recombinant Human TNFa

Recombinant Human TNF-alpha

230-00243-10
+

Source

Gene Symbols:
  • TNFA
Species:
Human
Accession Number:
  • P01375
Gene ID:
  • 7124
Expressed Region:
Val77-Leu233
Protein Name & Synonyms:
TNFa, TNF-alpha, Tumor necrosis factor

Preparation

Expression System:
E.Coli
Conjugation/Tag:
C-terminal 6x histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
17

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS (pH 7.4).
Concentration:
Lot specific (see the label on the vial)
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Preservative:
None
SDS-PAGE Image

Data Images

Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243). After stimulation with TNF-α, activated NF-κB p65 is translocated into the nucleus where it binds with its corresponding DNA. A. Western blotting result of NF-κB p65 from cytoplasm and nuclear fractions; B. Transcription factor activity assay of NF-κB p65 from nuclear fractions, measured by TF-ELISA kit (RayBio Cat# TFEH-p65).

Figure 1

Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243). After stimulation with TNF-α, activated NF-κB p65 is translocated into the nucleus where it binds with its corresponding DNA. A. Western blotting result of NF-κB p65 from cytoplasm and nuclear fractions; B. Transcription factor activity assay of NF-κB p65 from nuclear fractions, measured by TF-ELISA kit (RayBio Cat# TFEH-p65).

Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243) with the specific competitor or non-specific competitor. The result shows specific binding of NF-κB p65 to the NF-κB DNA binding site.

Figure 2

Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells or HeLa cells treated with TNF-α (RayBio Cat# 230-00243) with the specific competitor or non-specific competitor. The result shows specific binding of NF-κB p65 to the NF-κB DNA binding site.

Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells treated with TNF-α (RayBio Cat# 230-00243) and purified NF-κB p50. No cross-reactivity has been observed between NF-κB p65 and p50.

Figure 3

Transcription factor activity assay of NF-κB p65 from nuclear extracts of HeLa cells treated with TNF-α (RayBio Cat# 230-00243) and purified NF-κB p50. No cross-reactivity has been observed between NF-κB p65 and p50.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.