Recombinant Human TNT / TNNT2

Recombinant Human Troponin T type 2 (cardiac), E.coli-derived

$90.92
230-00048-10
+

Source

Gene Symbols:
  • TNNT2
Species:
Human
Accession Number:
  • P45379
Gene ID:
  • 7139
Expressed Region:
Ser2-Lys298
Protein Name & Synonyms:
TnTc, Cardiac muscle troponin T, cTnT.

Preparation

Expression System:
E.Coli
Conjugation/Tag:
N-terminal 6x histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
The predicted molecular weight is 36.5 kDa. The actual recombinant protein migrates as approximately 45 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 µmicro,m filtered solution in 0.1 M NaHCO3, 0.5 M NaCl, pH 7.5. Protein concentration prior to lyophilization was 0.25 mg/mL.
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers.
Endotoxin Level:
Not determined
Preservative:
None

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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