His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Lyophilized from a 0.2 um filtered solution in 10 mM acetic acid, pH 5.0.
Briefly spin the vial prior to opening to bring the contents to the vial bottom. Recombinant VEGF is acid-stable. It is recommended to reconstitute the lyophilized product with 0.1% trifluoroacetic (TFA) or 4 mM HCl to a concentration of 0.1-0.5 mg/mL.
Stability & Storage
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.