Recombinant Human VEGF-165 / VEGFA

Recombinant Human Vascular Endothelial Growth Factor A / VEGFA isoform l (165 aa)

230-00012-10
+

Source

Gene Symbols:
  • VEGF
Species:
Human
Accession Number:
  • NP_001165097
Expressed Region:
Ala27-Arg191
Protein Name & Synonyms:
Vascular endothelial growth factor, VEGF, vascular endothelial growth factor A, VEGF-A, VPF, vascular permeability factor.

Preparation

Expression System:
E.Coli
Conjugation/Tag:
N-terminal 6x histidine tag
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity:
> 95%
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
21

Protein Specifications

Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in 10 mM acetic acid, pH 5.0.
Reconstitution:
Briefly spin the vial prior to opening to bring the contents to the vial bottom. Recombinant VEGF is acid-stable. It is recommended to reconstitute the lyophilized product with 0.1% trifluoroacetic (TFA) or 4 mM HCl to a concentration of 0.1-0.5 mg/mL.
Endotoxin Level:
Not determined
Preservative:
None

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.