Recombinant Monkeypox Virus IMV Surface Membrane 14-kDa Fusion Protein, A29L

Recombinant monkeypox virus IMV surface membrane 14-kDa fusion protein (A29L) with a C-terminal His-tag, derived from the transfected HEK293 cells.
CODE:  230-30237

Product Specifications

Size:
100 ug
Format:
Liquid
Formulation:
Filtered solution in PBS with 1% mannitol and 5% trehalose
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific):
Bio-Rad protein assay reagent
Purity:
>95%
Endotoxin Level:
<0.5 EU per µg of the protein as determined by the LAL method
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications:
Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.

Source

Gene Symbols:
  • A29L
Species:
Monkeypox Virus (MPXV)
Accession Number:
  • Q90188
Expressed Region:
Met1-Glu110

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Tag:
His
Conjugation/Tag Notes:
C-terminal His-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of ∼13 kDa. Due to the abundant glycosylation, it migrates as two major bands: approximately ∼13 kDa non-glycosylated form and ∼16 kDa glycosylated form in SDS-PAGE under DTT and beta-mercaptoethanol reducing conditions.
SDS-PAGE
SDS-PAGE
Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.