Recombinant Mouse INSULIN-LIKE GROWTH FACTOR I

CODE:  DS-01-0487

Source

Species:
Mouse
Protein Name & Synonyms:
IGF-I,

Preparation

Purity:
>97% by SDS PAGE and HPLC
Molecular Weight (kDa):
7.6kD (70 amino acid sequence)

Protein Specifications

Format:
Purified recombinant protein - lyophilized
Concentration:
~1.0 mg/ml after reconstitution
Endotoxin Level:
<0.1 ng/ug
Preservative:
None present
Recommended Applications:
Indirect ELISA (0.2-0.4ng/well), Functional Assays (0.1-1.0ng/ml), Western Blotting (1.5-3.0ng/lane)

Description

DS-01-0487 is an E. coli-derived recombinant protein corresponding to full-length mature murine insulin-like growth factor 1 (IGF-1), a 70 amino acid peptide that is structurally and functionally related to insulin but with higher growth-promoting activities.

Recombinant mouse IGF-1 has an ED50 of <2.0ng/ml as determined by a cell proliferation assay using FDC-P1 cells.

Specifications

Size
10 ug

Stability & Storage

Storage/Stability
Prior to reconstitution store at +4°C. Following reconstitution store at -20°C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.