His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific):
Determined by BCA protein assay kit (Thermo Scientific)
Purity:
> 90%
Endotoxin Level:
Not determined
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Preservative:
None
Recommended Applications:
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array
Source
Gene Symbols:
MECA
Species:
Staphylococcus Aureus Subsp
Accession Number:
Q53707
Expressed Region:
Gly19-Glu668
Protein Name & Synonyms:
Penicillin binding protein 2
Preparation
Expression System:
E.Coli
Tag:
His
Conjugation/Tag Notes:
N-terminal 6x histidine tag
Molecular Weight (kDa):
74
SDS-PAGE Image
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.