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Recombinant S. aureus PBP2a

Recombinant Staphylococcus aureus Penicillin Binding Protein 2, expressed in E. coli

$256.74  
230-00051-100
+

Product Specifications

Size:
50 ug or 100 ug
Format:
Lyophilized powder
Formulation:
Lyophilized from a 0.2 um filtered solution in PBS.
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration:
Determined by BCA protein assay kit (Thermo Scientific)
Purity:
> 90%
Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level:
Not determined
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Preservative:
None
Recommended Applications:
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array

Source

Gene Symbols:
  • MECA
Species:
Staphylococcus Aureus Subsp
Accession Number:
  • Q53707
Expressed Region:
Gly19-Glu668
Protein Name & Synonyms:
Penicillin binding protein 2

Preparation

Expression System:
E.Coli
Conjugation/Tag:
N-terminal 6x histidine tag
Molecular Weight (kDa):
74
SDS-PAGE Image

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.