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Recombinant SARS-CoV-2 Nucleocapsid Protein
Recombinant SARS-CoV-2 Nucleocapsid Protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
<div class="text-right"><a href="/files/datasheet/230-30164.pdf" target="_blank" rel="noopener"><img src="/files/images/Buttons/pdf.png" alt="" /> Datasheet</a></div>
100 ug, 500 ug, or 1000 ug
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Lot specific (see the label on the vial), determined by BCA protein assay
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recombinant protein product has a calculated molecular mass of 47 kDa. Due to the abundant glycosylation, it migrates as approximately 55 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The minor small protein bands (25-30 kDa) likely the cleaved products. See deglycosylation analysis image below.
Stability & Storage
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.