Recombinant SARS-CoV-2 Omicron B.1.1.529 variant spike RBD protein

Recombinant SARS-CoV-2 Omicron variant spike S1 subunit protein RBD with 15 mutant sites (G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H) with C-terminal His-tag, derived from the transfected human HEK293 cells.

$312.90  
230-30201-100
+

Product Specifications

Size:
100 ug, 500 ug, or 1000 ug
Format:
Liquid
Formulation:
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Purity:
>95%
Endotoxin Level:
< 0.5 EU per µg of the protein as determined by the LAL method
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications:
Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.

Source

Gene Symbols:
  • S
Species:
SARS-CoV-2, Omicron Variant B.1.1.529
Accession Number:
  • QHD43416
Expressed Region:
Arg319-Phe541. Omicron 15 mutant sites (G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)
Protein Name & Synonyms:
Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)

Preparation

Expression System:
Human embryonic kidney 293 (HEK293) cells
Conjugation/Tag:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of ∼25 kDa. Due to the abundant glycosylation, it migrates as approximately ∼30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼25 kDa band.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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