Recombinant Sus scrofa (Pig) Erythropoietin (EPO), transfected HEK293 cell lysate

Recombinant Sus scrofa (Pig) Erythropoietin overexpression cell lysate, derived from the transfected HEK293 cells. (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (Catalog # 230-10006) transfected with empty expression vector.

$146.01
230-10186-100
+

Source

Species:
Sus scrofa (Pig)

Preparation

Expression System:
Human Embryonic Kidney 293 Cells
Conjugation/Tag:
N-terminal histidine tag
Purity:
Unpurified cell lysate

Protein Specifications

Reconstitution:
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.

Protein Name

Erythropoietin

Synonyms

Erythropoietin

Species

Sus scrofa (Pig)

Source

Expression System
Human Embryonic Kidney 293 (HEK293) Cells
Gene Symbol
EPO
Uniprot Accession #
NCBI Gene ID
397249
Expressed Region
Ala27-Arg194
Conjugation/Tag
N-terminal histidine tag
Molecular Mass (kDa)
Recombinant protein has a calculated molecular weight of about 19 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

Preparation

Preparation
Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.
Purity
Unpurified cell lysate
Purity determined by
Not applicable

Specifications

Format
Lyophilized powder
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Concentration
Determined by Bio-Rad protein assay reagent
Preservatives
None
Endotoxin Level
Not determined

Recommended Applications

Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein functional assay, protein-protein interaction, post-translational modifications, etc.

Stability & Storage

Shipping
Ice packs. Including one vial of HEK293 cell lysate transfected with empty expression vector.
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.  For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.