His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
Recombinant protein has a calculated molecular weight of about 16 kDa. Due to the abundant glycosylation, it migrates as approximately 18-25 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Lyophilized from a 0.2 µm filtered solution in PBS
Determined by Bio-Rad protein assay reagent
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Stability & Storage
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C.Ã For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.