RTK Phosphorylation Array

Semi-quantitative, Sandwich-based Array for Detecting RTK Phosphorylation Signatures

The RayBio® Human RTK Phosphorylation Antibody Array is a rapid, convenient and sensitive assay for simultaneous detection of phosphorylation of 71 different human tyrosine kinase receptors (RTKs). This assay allows the researcher to monitor pathway activation in cells or tissues without the excess time and effort of immunoprecipitation or Western blot.

The Human RTK Phosphorylation Array is spotted with a panel of 71 RTK-specific antibodies to capture the tyrosine kinases of interest. A biotinylated anti-phosphotyrosine antibody is then utilized to detect relative levels of activation in each captured protein. The array signals can be developed by chemiluminescence (membrane-based format), or fluorescence (glass slide-based format).  

For a comparison & overview of all phosphorylation assays, click here.

 
  Human RTK Phosphorylation Array C1 (AAH-PRTK-1)
C-Series Arrays - Semi-Quantitative, Sandwich-Based, Membrane Antibody Arrays
Detects 71 Human RTKs:
ABL1, ACK, ALK-1, Axl, Blk, BMX, Btk, Csk, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2, Fgr, FRK, Fyn, Hck, HGFR, IGF-1 R, Insulin R (CD220), Itk, JAK1, JAK2, JAK3, LCK, LTK, Lyn, MATK, M-CSF R, MUSK, NGFR (TNFRSF16), PDGFRA, PDGFRB, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R (CD117/c-kit), SRMS, SYK, Tec, Tie-1, Tie-2, TNK1, TRKB, TXK, Tyk2, TYRO10 (DDR2/TKT), VEGFR2, VEGFR3, ZAP70
Manual         C-Series Overview
 
  Human RTK Phosphorylation Array G1 (AAH-PRTK-G1)
G-Series Arrays - Semi-Quantitative, Sandwich-Based, Glass Slide Antibody Arrays
Detects 71 Human RTKs:
ABL1, ACK, ALK-1, Axl, Blk, BMX, Btk, Csk, Dtk, EGFR, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ErbB2, ErbB3, ErbB4, FAK, FER, FGFR1, FGFR2, FGFR2, Fgr, FRK, Fyn, Hck, HGFR, IGF-1 R, Insulin R (CD220), Itk, JAK1, JAK2, JAK3, LCK, LTK, Lyn, MATK, M-CSF R, MUSK, NGFR (TNFRSF16), PDGFRA, PDGFRB, PYK2, RET, ROR1, ROR2, ROS, RYK, SCF R (CD117/c-kit), SRMS, SYK, Tec, Tie-1, Tie-2, TNK1, TRKB, TXK, Tyk2, TYRO10 (DDR2/TKT), VEGFR2, VEGFR3, ZAP70
Manual         G-Series Overview

 

Features

Contents of kit

  • Detect activation of 71 tyrosine kinases in a single experiment
  • Minimal hands-on time: convenient, semi-quantitative
  • No immunoprecipitations, Western blots, or radioactive materials needed
  • No special equipment required
  • Chemiluminescent or fluorescent readout
  • Work reliably with wide range of cell lines 
  • High sensitivity and specificity
  • Affordable and cost effective for routine use
  • Antibody array membranes or glass slides
  • 2X Cell Lysis Buffer
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail
  • Blocking Buffer 
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotin-Conjugated Anti-Phosphotyrosine 
  • Streptavidin-Conjugated HRP or fluor
  • Detection Buffers (membrane array kits only)

 

How It Works

RTK Phosphorylation Array

 

 

After a blocking step, treated or untreated cell lysate is added into antibody array (either membrane or glass slide). The arrays are washed and biotinylated anti-phosphotyrosine antibody is added. After incubation with streptavidin-conjugated HRP or fluor, the signals are then visualized by chemiluminescence or fluorescence imaging system.

 
For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services.

 

Research Applications

  • Monitoring  pathway activation, protein kinase activity
  • Screening the effects of various treatments, activators or inhibitors in cell signaling, development and growth
  • Identifying crucial factors involved in disease processes
  • Discovering biomarkers for disease management
  • Identifying potential molecular targets for drug development
  • Identifying the molecular mechanisms of drug action
  • Discovering expression patterns for molecular classification of diseases
 

Reference List

  1. Acquired resistance to sunitinib in human renal cell carcinoma cells is mediated by constitutive activation of signal transduction pathways associated with tumour cell proliferation
    Species: Human
    Sample Type: Conditioned Media

  2. Metastasis-focused cell-based SELEX generates aptamers inhibiting cell migration and invasion. Int J Cancer. 2011 15 February;128(4):797?804.
    Species: Hamster
    Sample Type: Cell lysate

  3. Wenqing Q, Cooke LS, Stejskal A, Riley C, Croce KD, Saldanha JW, Bearss D, Mahadevan D. MP470, a novel receptor tyrosine, in combination with Erlotinib inhibits the HER family/PI3K/Akt pathway and tumor growth in prostate cancer. BMC Cancer 2009;9:142.
    Species: Human
    Sample Type: Cell lysate