Sandwich ELISA Kits

Quantitative Sandwich ELISAs for Protein Detection in a Variety of Sample Types

Sandwich ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of soluble proteins in biological fluids. RayBiotech sandwich ELISA kits are manufactured in Peachtree Corners, GA, fully validated, and backed by a 100% satisfaction guarantee. Under a large and highly experienced team of R&D scientists, our sandwich ELISA catalog has grown to cover 3000 target analytes and 16 species. When you buy directly from RayBiotech and our authorized distributors, you will never pay 3rd party markups, giving you access to the most competitive prices available. This, along with 3000+ literature citations, has made RayBio® sandwich ELISA kits some of the most trusted in the industry.



Antibody screening has important public health uses in determining immunity to and prevalence of COVID-19, as well as identifying who has been infected and evaluating potential blood based therapeutics. Detect IgG, IgM, and IgA antibodies to COVID-19 N and S1 RBD proteins in serum, plasma, and dried blood samples using the COVID-19 Indirect ELISA kits. Additionally, use the sandwich ELISAs for detecting N- and S-Proteins.

How It Works

Sandwich ELISA Detection

RayBio® sandwich ELISAs employ specific capture antibodies coated on a 96-well plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the biotin-labeled detection antibody is then added. After washing away the unbound biotinylated antibodies, HRP-conjugated streptavidin is pipetted to the wells, followed by a colorimetric substrate solution. The intensity of color development in the wells is proportional to the amount of target protein bound.


  • Excellent for follow up/confirmation of antibody array data
  • Pre-coated 96 well plate
  • Strip plate format minimizes waste
  • Complete kit includes all necessary reagents
  • Highly sensitive and specific
  • Reproducible and reliable
  • Optimized for serum, plasma, urine and cell culture medium
  • Hundreds of choices

Contents of Kit

  • 96-well pre-coated strip plate (12x8)
  • Wash Buffer Concentrate (20x)
  • Standard
  • Sample Diluent Buffers
  • Biotin-labeled Detection Antibody
  • HRP-Streptavidin concentrate
  • TMB One-Step Substrate Reagent
  • Stop Solution
  • Cell Lysis Buffer (for cell/tissue lysate kits only)

Why RayBiotech ELISA Kits?

  • We manufacture, not resell, ELISA kits. Right here in the USA.
  • Our ELISAs are ISO certified and validated for exceptional reproducibility. Read more about our quality control processes.
  • All ELISAs are backed by our 90-day 100% satisfaction guarantee.
  • Discounts and incentives available for publications citing our products, and for product reviews.


Reduce your time spent running ELISA without losing accuracy or spending more money. The RayBio SpeedELISA kits feature a simple, compressed workflow with only 1 wash step, and are available for all sandwich ELISA analytes.


  • Wozniak JM, Mills RH, Olson J, Caldera JR, Sepich-Poore GD, Carrillo-Terrazas M, Tsai CM, Vargas F, Knight R, Dorrestein PC, Liu GY, Nizet V, Sakoulas G, Rose W, Gonzalez DJ. Mortality Risk Profiling of Staphylococcus aureus Bacteremia by Multi-omic Serum Analysis Reveals Early Predictive and Pathogenic Signatures. Cell. 2020 Sep 3;182(5):1311-1327.e14. doi: 10.1016/j.cell.2020.07.040. PMID: 32888495; PMCID: PMC7494005. [view publication]
    Product: Human Fetuin B ELISA | Human IGFBP-3 ELISA | Human Serpin D1 ELISA
  • Grubišić V, McClain JL, Fried DE, Grants I, Rajasekhar P, Csizmadia E, Ajijola OA, Watson RE, Poole DP, Robson SC, Christofi FL, Gulbransen BD. Enteric Glia Modulate Macrophage Phenotype and Visceral Sensitivity following Inflammation. Cell Rep. 2020 Sep 8;32(10):108100. doi: 10.1016/j.celrep.2020.108100. PMID: 32905782; PMCID: PMC7518300. [view publication]
    Product: Human M-CSF ELISA
  • Wang P., Ji Y., Zhang X., et al. Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates. Nat Med. 2017 Apr;23(4):439-449. doi: 10.1038/nm.4290. [view publication]
    Product: Mouse IL-1 beta ELISA
  • Hong-Tae Park [view publication]
    Product: Bovine IL-17A ELISA
  • Day, Drew B., et al. "Association of ozone exposure with cardiorespiratory pathophysiologic mechanisms in healthy adults. "JAMA internal medicine 177.9 (2017): 1344-1353. [view publication]
    Product: Human vWF ELISA
  • Wu T., Ding H., Han J., et al. Antibody-Array-Based Proteomic Screening of Serum Markers in Systemic Lupus Erythematosus: A Discovery Study. J Proteome Res. 2016 Jul 1;15(7):2102-14. doi: 10.1021/acs.jproteome.5b00905. [view publication]
    Product: Human Siglec-5 ELISA | Human Osteopontin (OPN) ELISA | Human MMP-10 ELISA | Human IGFBP-2 ELISA | Human sICAM-1 ELISA | Human GDF-15 ELISA | Human Furin ELISA | Human Fas ELISA | Human sTNFRII ELISA | Human sTNFRI ELISA
  • Lim Y., Yoo J., Kim M., et al. GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands. Mol Cancer Ther. 2016 Feb;15(2):251-63. doi: 10.1158/1535-7163.MCT-15-0679 [view publication]
    Product: Human HB-EGF ELISA | Human TGF-alpha ELISA | Human EGF ELISA | Human BTC ELISA | Human Amphiregulin ELISA
  • Zanotto-Filho A, Rajamanickam S, Loranc E, Masamsetti VP, Gorthi A, Romero JC, Tonapi S, Gonçalves RM, Reddick RL, Benavides R, Kuhn J, Chen Y, Bishop AJR. Sorafenib improves alkylating therapy by blocking induced inflammation, invasion and angiogenesis in breast cancer cells. Cancer Lett. 2018 Jul 1;425:101-115. doi: 10.1016/j.canlet.2018.03.037. Epub 2018 Mar 30. PMID: 29608984; PMCID: PMC5930000. [view publication]
    Product: Human MMP-3 ELISA | Human MMP-1 ELISA
  • Han Y, Xu X, Tang C, Gao P, Chen X, Xiong X, Yang M, Yang S, Zhu X, Yuan S, Liu F, Xiao L, Kanwar YS, Sun L. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biol. 2018 Jun;16:32-46. doi: 10.1016/j.redox.2018.02.013. Epub 2018 Feb 15. Erratum in: Redox Biol. 2019 Jun;24:101216. PMID: 29475133; PMCID: PMC5842313. [view publication]
    Product: Human IL-1 beta ELISA | Human IL-18 ELISA | Human TGF-beta 1 ELISA
  • Pecorelli A, Cordone V, Messano N, Zhang C, Falone S, Amicarelli F, Hayek J, Valacchi G. Altered inflammasome machinery as a key player in the perpetuation of Rett syndrome oxinflammation. Redox Biol. 2020 Jan;28:101334. doi: 10.1016/j.redox.2019.101334. Epub 2019 Oct 6. PMID: 31606551; PMCID: PMC6812177. [view publication]
    Product: Human IL-18 ELISA
  • Dai Y, Wu X, Dai D, Li J, Mehta JL. MicroRNA-98 regulates foam cell formation and lipid accumulation through repression of LOX-1. Redox Biol. 2018 Jun;16:255-262. doi: 10.1016/j.redox.2018.03.003. Epub 2018 Mar 8. PMID: 29549823; PMCID: PMC5952997. [view publication]
    Product: Mouse LOX-1/OLR1 ELISA
  • Xing W., Xiao Y., Lu., et al. GFI1 downregulation promotes inflammation-linked metastasis of colorectal cancer. Cell Death Differ. 2017 May; 24(5): 929-943. [view publication]
    Product: Human TGF-beta 1 ELISA | Human TGF-beta 2 ELISA
  • Linder M., Hecking M., Glitzner E., et al. EGFR controls bone development by negatively regulating mTOR-signaling during osteoblast differentiation. Cell Death Differ. 2018 Feb 14. doi: 10.1038/s41418-017-0054-7 [view publication]
    Product: Mouse IGF-2 ELISA

More citations are listed per product on the individual product pages.