Sandwich ELISA Kits

How It Works

RayBio^® sandwich ELISAs employ specific capture antibodies coated on a 96-well plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the biotin-labeled detection antibody is then added. After washing away the unbound biotinylated antibodies, HRP-conjugated streptavidin is pipetted to the wells, followed by a colorimetric substrate solution. The intensity of color development in the wells is proportional to the amount of target protein bound.

Why RayBiotech ELISA Kits?

• We manufacture, not resell, ELISA kits. Right here in the USA.
• Our ELISAs are ISO certified and validated for exceptional reproducibility. Read more about our quality control processes.
• All ELISAs are backed by our 90-day 100% satisfaction guarantee.
• Discounts and incentives available for publications citing our products, and for product reviews.

Citations

• Cardenas E., Breaux K., Da Q., et ai. Platelet Munc13-4 regulates hemostasis, thrombosis and airway inflammation. Haematologica. 2018 Apr 19. pii: haematol.2017.185637. doi: 10.3324/haematol.2017.185637. [Epub ahead of print] [view publication]
  Product: Mouse PF-4 ELISA
  Sample Type: Platelet Releasate
• Chandan R., Banerjee R. Pro-apoptotic liposomes-nanobubble conjugate synergistic with paclitaxel: a platform for ultrasound responsive image-guided drug delivery. Sci Rep. 2018 Feb 8;8(1):2624. doi: 10.1038/s41598-018-21084-8. [view publication]
  Products: Mouse IFN-gamma ELISA | Mouse IL-6 ELISA | Mouse TNF-alpha ELISA
  Sample Type: Conditioned Media
• Chen D., Chen Y., Chen H., et al. Human parvovirus B19 nonstructural protein NS1 activates NLRP3 inflammasome signaling in adult‑onset Still's disease. Mol Med Rep. 2018 Feb;17(2):3364-3371. doi: 10.3892/mmr.2017.8275. [view publication]
  Product: Human IL-1 beta ELISA
  Sample Type: Conditioned Media
• Dey S., Bishayi B. Killing of S. aureus in murine peritoneal macrophages by Ascorbic acid along with antibiotics Chloramphenicol or Ofloxacin: Correlation with inflammation. Microb Pathog. 2018 Feb;115:239-250. doi: 10.1016/j.micpath.2017.12.048 [view publication]
  Products: Mouse IFN-gamma ELISA | Mouse IL-10 ELISA | Mouse IL-6 ELISA | Mouse TNF-alpha ELISA
  Sample Type: Conditioned Media
• Etulain J., Mena H., Meiss R., et al. An optimised protocol for plateletrich plasma preparation to improve its angiogenic and regenerative properties. Scientific Reports (2018) 8:1513 | DOI:10.1038/s41598-018-19419-6 [view publication]
  Product: Human VEGF-A ELISA
  Sample Type: Conditioned Media
• Habashy N., Abu Serie M., Attia W., Abdelgaleil S. Chemical characterization, antioxidant and anti-inflammatory properties of Greek Thymus vulgaris extracts and their possible synergism with Egyptian Chlorella vulgaris. Journal of Functional Foods , Volume 40, January 2018, Pages 317-328, doi.org/10.1016/j.jff.2017.11.022 [view publication]
  Product: Human TNF-alpha ELISA
  Sample Type: Conditioned Media
• Li X., Yan X., Wang Y., et al. NLRP3 inflammasome inhibition attenuates silica-induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells. Exp Cell Res. 2018 Jan 15;362(2):489-497. doi: 10.1016/j.yexcr.2017.12.013. [view publication]
  Product: Human IL-18 ELISA
  Sample Type: Conditioned Media
• Linder M., Hecking M., Glitzner E., et al. EGFR controls bone development by negatively regulating mTOR-signaling during osteoblast differentiation. Cell Death Differ. 2018 Feb 14. doi: 10.1038/s41418-017-0054-7 [view publication]
  Product: Mouse IGF-2 ELISA
  Sample Type: Conditioned Media
• Mendt M., Kamerkar S., Sugimoto H., et al. Generation and testing of clinical-grade exosomes for pancreatic cancer. JCI Insight. 2018 Apr 19;3(8). pii: 99263. doi: 10.1172/jci.insight.99263. [view publication]
  Product: Mouse IL-6 ELISA
  Sample Type: Serum
• Mohamed AAR, Elbohi KM, Sharkawy NIE and Hassan MA. Biochemical and Apoptotic Biomarkers as Indicators of Time Elapsed Since Death in Experimentally Induced Traumatic Brain Injury. Beni-Suef University Journal of Basic and Applied Sciences, doi.org/10.1016/j.bjbas.2018.01.001. [view publication]
  Product: Rabbit IL-1 beta ELISA
  Sample Type: Serum
• Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR [view publication]
  Products: Human IFN-gamma ELISA | Human IL-10 ELISA | Human IL-12 p70 ELISA | Human IL-4 ELISA | Human MCP-1 ELISA | Human TNF-alpha ELISA
  Sample Type: Plasma
• Schumann C., Nguyen D., Norgard M., et al. Increasing lean muscle mass in mice via nanoparticle-mediated hepatic delivery of follistatin mRNA. Theranostics. 2018; 8(19): 5276–5288. Published online 2018 Oct 22. doi: 10.7150/thno.27847 [view publication]
  Products: Human Follistatin ELISA | Human GDF-8/Myostatin ELISA | Mouse Activin A ELISA
  Sample Type: Conditioned Media

More citations are listed per product on the individual product pages.