This RayBio® diagnosis kit utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP), which amplifies SARS-CoV-2 RNA without the need for thermal cycling. This is accomplished using specialized primers that enable continuous amplification of nucleic acids at 60°C. RT-LAMP is faster than traditional RT-PCR with a colorimetric endpoint readout. Thus, detection of viral RNA can be performed by eye or with a plate reader capable of reading absorbance at 440 nm and 560 nm.
This kit uses six primer-probe sets specific to the SARS-CoV-2 gene encoding for the nucleocapsid protein. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.
Absence of viral RNA is represented by a pink color (560 nm)(see picture above). The presence of viral RNA is represented by a yellow color (440 nm). An OD ratio of 440 nm / 560 more than 1.4 indicates that SARS-CoV-2 viral RNA is present.
|Component||Ingredients||Volume (per 25 tests)|
|PCR Reaction Solution||RayBio® SARS-CoV-2 RNA RT-LAMP Colorimetric Master Mix||375 μl|
|Positive Control||SARS-CoV-2 Synthetic Nucleocapsid RNA (1000 copies/µl)||10 µL|
|Negative Control||Ultra-pure DEPC Water||2 mL|
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