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SARS-CoV-2 RNA Isothermal Amplification Colorimetric Assay Kit

25, 100, or 500 tests. No PCR instrument required! Suitable for the qualitative detection of SARS-CoV-2 virus in human throat swabs and alveolar lavage. Results detected by eye or plate reader.

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RT-LAMP-25

Antigen Information

Protein Name & Synonyms:
Gene encoding for the Nucleocapsid (N) protein

Assay Format

Sensitivity:
25 viral RNA copies / µL
Compatible Sample Types:
  • Broncheoalveolar lavage
  • Throat swab
Method of Detection:
Reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Quantitative/Semi-Quantitative:
  • Qualitative (by eye) & Semi-Quantitative (with plate reader)

Product Specifications

Size:
25, 100, or 500 tests
Number of Targets Detected:
6 regions specific to the SARS-CoV-2 N gene. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.
Plate Reader Compatibility:
Absorbance at 440 nm and 560 nm

Introduction

This RayBio® diagnosis kit utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP), which amplifies SARS-CoV-2 RNA without the need for thermal cycling. This is accomplished using specialized primers that enable continuous amplification of nucleic acids at 60°C. RT-LAMP is faster than traditional RT-PCR with a colorimetric endpoint readout. Thus, detection of viral RNA can be performed by eye or with a plate reader capable of reading absorbance at 440 nm and 560 nm.

This kit uses six primer-probe sets specific to the SARS-CoV-2 gene encoding for the nucleocapsid protein. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.

Absence of viral RNA is represented by a pink color (560 nm)(see picture above). The presence of viral RNA is represented by a yellow color (440 nm). An OD ratio of 440 nm / 560 more than 1.4 indicates that SARS-CoV-2 viral RNA is present.

Kit Components

Component Ingredients Volume (per 25 tests)
PCR Reaction Solution RayBio® SARS-CoV-2 RNA RT-LAMP Colorimetric Master Mix 375 μl
Positive Control SARS-CoV-2 Synthetic Nucleocapsid RNA (1000 copies/µl) 10 µL
Negative Control Ultra-pure DEPC Water 2 mL

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