Serpin Peptidase Inhibitor, Clade B (Ovalbumin) Member 4, LEUPIN, SCCA2, Squamous Cell Carcinoma Antigen 2, Protease Inhibitor (Leucine-Serpin), Serine (Or Cysteine) Proteinase Inhibitor Clade B (Ovalbumin) Member 4, SCCA2/SCCA1 Fusion Protein, Peptidase Inhibitor 11, Serpin B4, PI-11, PI11, Leupin, SCCA-2
Human Embryonic Kidney 293 Cells
N-terminal 6x histidine tag
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa):
The recombinant human SCCA2 has a calculated molecular mass of 47 kDa. Due to the abundant glycosylation, it migrates as approximately 55 and 60 kDa protein bands (confirmed by Western blotting) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Lyophilized from a 0.2 µ,m filtered solution in PBS
Determined by BCA protein assay kit (Thermo Scientific)
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Stability & Storage
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.