Sheep Anti-Human ALPL

Sheep Anti-Human ALPL

DS-PB-03001
+

Antigen Information

Protein Name & Synonyms:
ALPL
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 1/1500, 1/15000)
Concentration:
IgG concentration 5.0mg/ml
Preparation:
Antisera to human alkaline phosphatase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.
Species Reactivity:
Human

Specificity

This product recognizes human bone alkaline phosphatase (BAP), a membrane-bound hydrolase enzyme expressed by osteoblast cells.

Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). Although the exact biochemical function of BAP is uncertain, measurement of the serum levels of BAP can be used as a biochemical indicator of bone turnover. Conditions which present with a decrease in the level of BAP (hypophosphatasemia) include the inherited bone-deforming disorder hypophosphatasia and osteoporosis, whilst an increase in BAP (hyperphosphatasemia) is associated with Paget❏'s disease, bone fractures and osteosarcomas.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to human alkaline phosphatase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.

Immunogen:
Alkaline phosphatase from human bone
Preservative:
0.09%Sodium Azide (NaN3)

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.